Data Analysis?Photograph the destained gel on an illuminated box each.?Create a standard curve of the protein standards plotting log of molecular weightagainst distance moved from the wells.?Usi

Data Analysis?Photograph the destained gel on an illuminated box each.?Create a standard curve of the protein standards plotting log of molecular weightagainst distance moved from the wells.?Using a standard curve of the protein standards calculate the size of the GFPpurified in the:oColumn purification samplesoIn the cell pellet lysates of the lb/ara/amp. You are only going to size theband(s) that is darker in the lb/ara/ amp sample.5oPrepare samples of each of the samples (tube 1,2,3 and lysate if you have it).oHeat all the samples at 95oC for 10minsCell PelletsoYou will have two pellets from lab 9: ara and ara/ampoAdd buffer to each tube as belowComponentsVolumePelletNUPAGE LDS Sample buffer200ulTotal volume200uloAliquot 20 ul of ara and ara/amp two separate tubes labeled ‘heat’oAliquot 20 ul of ara and ara/amp two separate tubes labeled ‘no-heat’oIncubate the ‘heat’ samples for 10 minutes at 95oCSample LoadingoUse the pipette equipped with a protein gel loading tip to underlay the samples intothe gel wells (see figure below).oSlowly draw the sample up into the tip as it will take time to fill due to the narrowbore.oLower the tip to the bottom of the sample well and slowly pipet sample into wellwithout contaminating another well with the sample.Gel 1 – Samples for gel lanesoProtein ladder – 5uloTube